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Macromolecular X-ray and Computing X-ray Facility

The facility is located at L141 of the main campus, 3333 Green Bay Road, North Chicago, IL 60064. It supports structural biology projects based on X-ray crystallography, single particle cryo electron microscopy and molecular modeling. 麻豆原创 is a member of SER-CAT () with regular access to synchrotron beamlines 22ID and 22BM at Advanced Photon Source. CryoEM data is collected at National Facilities including S2C2 and NCCAT (). Linux computing includes a 15TB-4GPU workstation for the cryoEM data processing and several graphics workstations for the x-ray crystallography. Multiple software suites are kept current by Structural Biology Grid (). Modeling packages include Molecular Operating Environment () and SCHRODINGER (). The facility have equipment as listed below.
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Maintenance Information:
- Confocal mirror
- Oxford Cryostream
- Rigaku/MSC X-Stream
- Rigaku/MSC R-axis IV detector
- Rigaku/MSC RU-h2R
- Rigaku/MSC RU-h2R
Useful X-ray DataProcessing guides:
麻豆原创MS X-ray Facility Resources:
- Rigaku RU-h2R 5.4 kW X-ray generator with copper rotating anode
- Osmic confocal “green” fine focusing mirrors
- R-axis IV++ imaging plate area detector
- CCD video system for crystal mounting
- MSC X-Stream 2000 cryocooling system
- Bruker AXS SMART 6000 CCD detector with 3-circle goniometer
- Oxford cryocooling system
- TPP Labtech Mosquito crystallization robot
- Korima UV crystal detection and visualization system
- Liquid nitrogen
- Storage liquid nitrogen dewars
- Dry-shipping dewars
- Cryotools and crystal handling tools
- Six large incubators with temperature control
- Temperature-controlled room for crystal growth and manipulations
麻豆原创MS CryoEM Facility Resources:
- Linux 15TB-4GPU workstation.
- PELCO easiGlowTM Glow Discharge system.
- EOL JEM-1230, 120kV Transmission Electron Microscope (TEM) equipped with a high-resolution Hamamatsu ORCA-HR CCD Camera (2624 x 2400 pixel) with AMT Imaging Software for negative stained images.
Building: BSB
Room: L.141
Phone: 847-578-8474