The VITROS slides are dry, multilayered analytical elements coated on polyester supports. A small amount of patient sample is deposited onto the slide and evenly distributed to all layers. The spreading layer contains the appropriate substrate and other components needed for the reaction. The analyte in the sample catalyzes a reaction sequence that produces products which absorb light at wavelengths between 340–680 nm. The change in reflectance density is measured by spectrophotometry and is proportional to the analyte concentration in the specimen. Test methodologies include colorimetric, enzymatic end-point, two-point or multi-point rate, and potentiometric assays.

Store/transport at 15–30°C for ≤8 hours; 2–8°C for ≤48 hours; freeze at ≤ –20°C if delayed. Avoid repeated freeze–thaw cycles.

Hemolyzed, lipemic, or unseparated specimens.

Dry Slide Chemistry

Uric Acid

• Female:
• 17–34 years: 2.5–6.2 mg/dL
• 35–44 years: 2.5–7.0 mg/dL
• 44 years: 2.5–7.5 mg/dL
• Male: 3.5–8.5 mg/dL

Iron

• Female: 37–170 µg/dL
• Male: 49–181 µg/dL

Total Iron Binding Capacity (TIBC)

• Female: 265–497 µg/dL
• Male: 261–462 µg/dL

LDH

• 313–618 U/L

γGT

• 12–58 U/L

• Johnson & Johnson Clinical Diagnostics, Inc. VITROS Chemistry Products: Test Methodology. August 1997.

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