This test quantitates and analyzes cell-surface markers of lymphocyte subsets and other leukocyte populations using monoclonal antibodies. The two major classes of lymphocytes are T lymphocytes, which mediate cellular immunity, and B lymphocytes, which mediate humoral immunity. Monoclonal antibodies specific to these cell types and their subsets are conjugated to fluorochromes and incubated with lymphocytes. The percentage of positively stained cells is then determined by flow cytometry. Results are reported as the percentage of positive lymphocytes; absolute counts are additionally provided for the immunodeficiency profile.

Send at room temperature. Do not refrigerate.

• Refrigerated or cold specimens (including those shipped on ice)
• Extensive clotting or hemolysis
• Specimens older than 48 hours

Deliver to the laboratory within 24 hours.

Flow Cytometry.

Refer to laboratory report.

• CD3+CD8+
• CD3+CD4+
• CD3+CD56+
• CD19+

• Lovett, E.J. et al: Application of flow Cytometry to Diagnostic Pathology. Lab. Invest. 50:115, 1984.
•  Lane, H.C. and A. Fauci. Immunologic Aspects of the acquired immunodeficiency syndrome. Adv. Host Def. Mech. 5:131, 1985.

Back to Reproductive Immunology