HLA alleles are typed vis Next Generation Sequencing (NGS) technologies. Extracted DNA undergoes long range PCR, followed by NGS library preparation and subsequently loading onto the sequencer. The targeted nucleotide sequence of each loci is determined and then matched to HLA database (IMGT) to produce ultra-high resolution HLA typing. Therefore, typing with NGS substantially decreases ambiguity rates when compared to other current methods (SSO & SSP).

The NGS method is used to type HLA-Class II alleles. Some of these alleles have been associated with certain disease processes or clinical conditions. The DQ-Beta 2 allele may be associated with recurrent miscarriages in some couples.

Specimen should be delivered to the laboratory within 72 hours at room temperature.

Peripheral blood specimens that are clotted, have not been collected in EDTA, or frozen are not acceptable.

Next Generation Sequencing.

See lab report.

• DYNAL SSP General Protocol, June 1994,DYNAL Inc., Lake Success, N.Y.
• DYNAL DQB1 – SSP 1st Sets Product Description, Specificity and Interpretation Table, September 1994,DYNAL Inc., Lake Success, N.Y.
• Bender, et al. Clinical Laboratory Procedure Manual, NCCLS. Code GP2-A, Vol. No. 2.

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