The Human Leukocyte Antigen Class II (HLA D) genes are located on chromosome 6. The HLA D genes are organized into three regions: HLA DR, DQ and DP each of which encodes an alpha and beta glycopeptide. The complete DNA sequence of the HLA DQa alleles is known. The DQa alleles are detected Next Generation Sequencing (NGs) technologies. With NGS the targeted nucleotide sequence of each loci is determined and then matched to HLA database (IMGT) to produce ultra-high resolution HLA typing. Therefore, typing with NGS substantially decreases ambiguity rates when compared to other current methods (SSO & SSP).

Specimen should be delivered to the laboratory within 72 hours at room temperature.

Peripheral blood specimens that are clotted, have not been collected in EDTA, or frozen are not acceptable.

Next Generation Sequencing

See lab report

• Terasaki, P.I., Bernoco, F., Park, M.S., Ozturk, G., and Iwaki, Y. Microdroplet testing for HLAA, -B, -C and -D antigens. American Journal of Clinical Pathology 69:103-120, 1978.
• Bodmer J., Marsh S.. Albert E., Bodmer W., Bontrop R., Charron D., Dupont B., Erlich H., Mach B., Mayr W., Parham P., Sasazuki T., Schreuder G., Strominger J., Svejgaard A. and Terasaki P. Nomenclature for factors of the HLA system, 1995. Human Immunology 43:149-164, 1995.
• Puregene DNA Purification System, Genomic DNA Purification kit instruction by Gentra System of Minneapolis, MN.

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