Detection of antinuclear antibodies (ANA) against DNA and histones is an important diagnostic tool in the evaluation and management of rheumatic diseases. These autoantibodies react with various nuclear components, including polynucleotides, histones, nonhistone proteins, nucleolar antigens, and nuclear matrix compounds. Certain anti-DNA antibodies are considered marker antibodies for specific inflammatory rheumatic diseases, and different disease subsets may display distinct DNA antibody profiles. This Enzyme-Linked Immunosorbent Assay (ELISA) assay detects antibodies to the autoantigens double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), histones, and Scl-70 in human serum.

• Room temperature: up to 8 hours
• 2–8°C: up to 48 hours
• –20°C or below: for longer storage

Send frozen serum on dry ice. Do not freeze

Enzyme-Linked Immunosorbent Assay (ELISA)

Negative
Results are reported as negative, borderline or positive.

• dsDNA
• ssDNA
• Histones
• Scl-70

1. Reichlin M. Autoantibodies to nuclear antigens in systemic lupus erythematosus and related diseases. Rheumatic Disease Clinics of North America. 1992;18(2):369–387.
2. Mahler M, Fritzler M.J. The clinical significance o

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